effects of some alpha-amylase inhibitors on the salivary alpha-amylase activity of the stripped bug, graphosoma lineatum (heteroptera: scutelleridae)
نویسندگان
چکیده
abstract digestive enzyme inhibitors are proteinacious or nonproteinacious compounds which reduce an enzyme activity through attaching to its active site and/or its substrate. nowadays, plant enzyme inhibitors are of great importance because 1) these have considerable effects on insect digestive enzymes and as a result on their development; and 2) the transgenic plants expressing them are safe. in this study, the effects of nacl, ethylenediamine tetraacetate disodium dehydrate (edta), tris, sodium dodecyl sulfate (sds) and an alpha-amylase inhibitor derived from wheat kernels (waai) on alpha-amylase activity of adults of the stripped bug graphosoma lineatum (l.) during 60 minutes incubation was studied. distilled water was considered as the control. the results showed that the effects of the inhibitor type on adult alpha-amylase activity was significant (p<0.01). in general, enzyme activities in nacl, edta and tris were the highest (nearly 54% of the control) and there was not significant differences between them. enzyme activities in sds (nearly 31% of the control) and in waai (nearly 12.5% of the control) showed significant differences to each other and also to 3 previously mentioned compounds. at low concentrations of nacl, edta, tris and sds (1 and 2 mm) and at the beginning of incubation period, female alpha-amylase activity was greater than that of the males which shows that the female enzyme has had more resistance against the inhibitors.
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عنوان ژورنال:
حفاظت گیاهانجلد ۲۴، شماره ۲، صفحات ۰-۰
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